| α1BG

Alpha-1-B glycoprotein is a 54.3 kDa protein in humans that is encoded by the A1BG gene.The protein encoded by this gene is a plasma glycoprotein of unknown function. The protein shows sequence similarity to the variable regions of some immunoglobulin supergene family member proteins. Patients who have pancreatic ductal adenocarcinoma show an overexpression of A1BG in pancreatic juice.
 

| Typical data

As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, wash technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data are provided below for reference only.

 

Concentration (ng/mL)	100	50	25	12.5	6.25	3.12	1.56	0
OD	2.47	1.4	0.77	0.46	0.32	0.22	0.2	0.08
Corrected OD	2.39	1.32	0.69	0.38	0.24	0.14	0.12	-

 

| Repeatability

Intra-assay Precision (Precision within an assay)：CV% < 10%; Inter-assay Precision (Precision between assays)：CV% < 10%
 

| Recovery

The recovery of Human α1BG spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

 

Sample Type	Range(%)	Average Recovery(%)
Serum(n=8)	85-101	97
Plasma(n=8)	92-105	102

 

| Linearity

Samples were spiked with high concentrations of Human α1BG and diluted with Universal Diluent to produce samples with values within the range of the assay.

 

		Serum(n=4)	Plasma(n=4)
1 : 2	Range (%)	84-95	87-96
	Average (%)	91	93
1 : 4	Range (%)	89-104	87-108
	Average (%)	95	98